psa Search Results


cd56  (Miltenyi Biotec)
99
Miltenyi Biotec cd56
Cd56, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pisum sativum agglutinin  (Vector Laboratories)
93
Vector Laboratories pisum sativum agglutinin
Pisum Sativum Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psa antibodies  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology psa antibodies
Psa Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psat1  (Proteintech)
96
Proteintech psat1
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Psat1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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low noise amplifier  (Mini-Circuits)
92
Mini-Circuits low noise amplifier
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Low Noise Amplifier, supplied by Mini-Circuits, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biological chemistry 5877  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc biological chemistry 5877
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Biological Chemistry 5877, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prostate specific antigen psa  (Lee Biosolutions)
91
Lee Biosolutions prostate specific antigen psa
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Prostate Specific Antigen Psa, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti prostate specific antigen psa antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti prostate specific antigen psa antibody
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Anti Prostate Specific Antigen Psa Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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accubind total psa test  (Monobind)
93
Monobind accubind total psa test
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Accubind Total Psa Test, supplied by Monobind, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human psa  (R&D Systems)
92
R&D Systems recombinant human psa
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Recombinant Human Psa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psa ncam apc  (Miltenyi Biotec)
99
Miltenyi Biotec psa ncam apc
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Psa Ncam Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human psa protein  (Biosynth Carbosynth)
90
Biosynth Carbosynth human psa protein
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Human Psa Protein, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4. PSAT1 is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1.

doi: 10.1159/000430361

Figure Lengend Snippet: Fig. 4. PSAT1 is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.

Article Snippet: The following antibodies were used: p-GSK-3β, GSK-3β, Snail, E-cadherin, Vimentin (Cell Signaling Technology); and PSAT1 (Proteintech Group Inc, Chicago, IL).

Techniques: Binding Assay, Sequencing, Luciferase, Mutagenesis, Expressing, Western Blot